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DNA Lambda C1857 S7 (Deoxyribonucleic acid)
DNA Lambda C1857 S7 (Deoxyribonucleic acid)
Pricing
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| Isolated from heat-inducible lysogen C1857 S7. Digestion with EcoRI and HindIII generates the characteristic 7 and 5 bands. | | | Catalog # | D3910 | | Unit Definition | 1 A260 unit is equivalent to 50ug | | Quality Control | Contains no detectable exonuclease, endonuclease or ribonuclease. | | Western Blot | Lane 1 control uncleaved-1 ul | | Lane 2 EcoRI digest- 3 ul | | Lane 3 Hind III digest- 3 ul | | Lane 4 uncleaved- 0.5 ul | | Lane 5 uncleaved- 1.0 ul | | Lane 6 uncleaved- 2.0 ul | | Lane 7 EcoRI digest- 1 ul | | Lane 8 EcoRI digest- 3 ul | | Lane 9 Hind III digest- 1 ul | | Lane 10 Hind III digest- 3 ul | | Restriction Digest | 20ug of DNA Lambda C1857 S7 is incubated with 2ul of 20,000u/ml Hind III or EcoRI in a 40ul total volume for two hours at 37°C (2u/ug DNA). Sample volumes indicated in lane assignments (diluted with dye and water to 20ul volume) are then run on 0.8% agarose ethidium bromide gel @50mA 120-125 volts for 1 hour. | | CAS Number | 91080-14-7 | | Molecular Weight | 31.5kD | | Purity | Purified to an A260/A280 1.8 . | | Concentration | ~750ug/ml | | Form | Supplied as a liquid in 10mM Tris-HCl, pH 8.0, 1mM EDTA. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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