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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -DNA Ligase 1 (DNL1, LIG1, DNA Ligase I, Ligase I DNA ATP Dependent, MGC117397, MGC130025, Polydeoxyribonucleotide Synthase [ATP] 1) (Azide Free)

Anti -DNA Ligase 1 (DNL1, LIG1, DNA Ligase I, Ligase I DNA ATP Dependent, MGC117397,
MGC130025, Polydeoxyribonucleotide Synthase [ATP] 1) (Azide Free)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents.
Catalog #D3925-01J
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (paraffin): 1.5ug/ml
ELISA: 1ug/ml-3ng/ml
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10B2735
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to a portion of amino acids within aa810-920 of human DNA Ligase I.
SpecificityRecognizes human DNA Ligase I.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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