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D3925-01K Goat Anti-DNA Ligase 1 (DNL1, LIG1)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_000225.1
Shipping Temp
Blue Ice
Storage Temp
-20°C

Eukaryotic DNA ligases are ATP-dependent enzymes that catalyze the joining of single and double stranded DNA breaks, which is an essential step in DNA repair, replication and recombination. There are at least 4 DNA ligases, termed DNA ligase I-IV, have been cloned and characterized. DNA ligase-I is functionally homologous to the DNA ligase encoded by the S. cervisiae CDC9 gene. The joining of Okazaki fragments during lagging strand DNA replication is due to DNA ligase-I. DNA ligase-I (919 aa, chromosome 19q13.2-q13.3; mouse 916 aa, rat 919 aa) is a nuclear protein. Its deficiency causes immunodeficiency and cellular hypersensitivity of DNA-damaging agents.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:16,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-RVREDKQPEQATTS, from the internal region (near C-Terminus) of human LIG1/DNA Ligase I (NP_000225.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human LIG1/DNA Ligase 1. Sequence homology: rat
References
1. Ferrari G, Rossi R, Arosio D, Vindigni A, Biamonti G, Montecucco A, Cell cycle-dependent phosphorylation of human DNA ligase I at the cyclin-dependent kinase sites. J. Biol. Chem. 2003 Sep 278 (39): 37761-7.
USBio References
No references available
Conjugates
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