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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -DNA Ligase IV (DNA Ligase 4, DNA Ligase IV ATP Dependent, DNA Joinase, DNA Repair Enzyme, LIG4, Ligase IV, Ligase IV DNA ATP Dependent, Polydeoxyribonucleotide Synthase [ATP] 4, Polynucleotide Ligase, Sealase)

Anti -DNA Ligase IV (DNA Ligase 4, DNA Ligase IV ATP Dependent, DNA Joinase, DNA Repair
Enzyme, LIG4, Ligase IV, Ligase IV DNA ATP Dependent, Polydeoxyribonucleotide Synthase
[ATP] 4, Polynucleotide Ligase, Sealase)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
LIG4 encodes a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome.
Catalog #D3925-20C
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormPBS with 2% sucrose.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide derived from a region between residues 275 - 324 of human DNA Ligase IV.
SpecificitySpecies Crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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