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D3930-05 DNA Marker 50bp Ladder, 13 Fragments (50-1000bp), BioGenomics™ CAS:

Specifications
References
Brand
BioGenomics™
Grade
Molecular Biology Grade
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
Supplied with 6x Loading Dye Solution

DNA Marker 50bp Ladder is designed for sizing and approximate quantification of wide range double-stranded DNA on agarose and polyacrylamide gels. The ladder is composed of 13 chromato- graphically purified individual DNA fragments (in base pairs): 1000, 900, 800, 700, 600, 500, 400, 300, 250, 200, 150, 100, 50. Note: The bright bands at 500 and 250bp serve as the reference.

Concentration
0.5mg DNA/ml
Storage Buffer
10mM Tris-HCl (pH 7.6), 1mM EDTA
6X Loading Dye Solution
Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60mM EDTA.
Quality Control
Analysis of 0.5ug (1ul) of the DNA Ladder on agarose gel and 1ug (2ul)-on polyacrylamide gel by ethidium bromide staining generates 13 discrete band patterns.
Quantity of DNA in Band
Fragment (bp) % ng/0.5ug DNA 1000 6 30 900 6 30 800 6 30 700 6 30 600 6 30 500 15 75 400 6 30 300 6 30 250 15 75 200 7 35 150 7 35 100 7 35 50 7 35 Total 100 500
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
References
1. Stellwagen, N.C., Biochemistry, 22: 6,183-6,193 (1983). 2. Lane, D., et al., Microbiological Reviews, 56: 509-528 (1992). 3. Stellwagen, N.C., Electrophoresis, 21: 2,327-2,334 (2000).
USBio References
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