Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

T4 DNA Ligase catalyzes the joining of the 3I -OH and 5I phosphate termini of duplex DNA or RNA.
Catalog #	D3931-02
Applications	• Joining dsDNA with cohesive or blunt termini
	• Joining synthetic linkers to blunt-ended DNA
	• Repairing nicks in duplex DNA, RNA or DNA/RNA hybrids
CAS Number	9015-85-4
Molecular Weight	62kD
Source	E. coli
Storage Buffer
20mM Tris HCl, pH 7.5, 0.1mM EDTA, 1mM DTT, 50mM KCl and 50% glycerol
Reaction Buffer (10X)
400mM Tris HCl, 100mM MgCl2, 100mM DTT, 5mM ATP
Concentration	30u/ul
Unit Definition
One unit of enzyme will catalyze the exchange of 1 nanomole 32PPi (pyrophosphate) into a Norit®- absorbable compound in 20 minutes at 37°C.
Quality Control
SDS-PAGE
Contains no detectable endo-, exodeoxyribonucleases, ribonucleases.
Tested for the cohesive and blunt-ended DNA fragment joining.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	EC=6.5.1.1; E. coliSupplied with Reaction Buffer