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You are here:Home » Antibodies » Abs to Enzymes, Methyltransferase » Anti -DNA Methyltransferase 1 (DNMT, MCMT, M.Hsa1, DNA (cytosine-5-)-methyltransferase 1, DNA MTase Mmu1, M.HsaI)

Anti -DNA Methyltransferase 1 (DNMT, MCMT, M.Hsa1, DNA (cytosine-5-)-methyltransferase
1, DNA MTase Mmu1, M.HsaI)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
DNMT1 is a nuclear enzyme with a major role in the maintenance of DNA methylation patterns after replication. DNA methylation is significant for epigenetic regulation of gene expression, X chromosome inactivation, genomic imprinting, and development. Overexpression of DNMT1 has been detected in several human cancers.
Catalog #D3931-61G
Blocking Peptide D3931-61T
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:5001: 5,000
Immunoprecipitation: 2-4 ug/mg protein
Optimal dilution determined by the researcher.
Recommended Secondary ReagentsSheep anti rabbit IgG: HRP
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris-citrate/phosphate buffer, pH 7.5, 0.02% sodium azide, before the addition of glycerol to 40%.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to a region within human DNMT1 encoded within exon 25.
SpecificityRecognizes human DNA (cytosine-5) methyltransferase 1, also known as DNMT1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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