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You are here:Home » Antibodies » Abs to Enzymes, Methyltransferase » Anti -Dnmt1, phosphorylated (Ser714) (DNA (cytosine-5)-methyltransferase 1, Dnmt1, MCMT, DNA Methyltransferase HsaI, DNA MTase HsaI, M.HsaI, CXXC-type Zinc Finger Protein 9, AIM, CXXC9, DNMT)

Anti -Dnmt1, phosphorylated (Ser714) (DNA (cytosine-5)-methyltransferase 1, Dnmt1, MCMT,
DNA Methyltransferase HsaI, DNA MTase HsaI, M.HsaI, CXXC-type Zinc Finger Protein 9, AIM,
CXXC9, DNMT)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.
Catalog #D3931-63E
ApplicationsSuitable for use in ELISA and Dot Blot. Other applications not tested.
Recommended DilutionELISA: 1:1,000
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to amino acid residues surrounding Ser714 of human Dnmt1 (KLH).
SpecificityRecognizes human Dnmt1 when phosphorylated at Ser714.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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