You are here:Home
»
Antibodies
»
Abs to Enzymes, Methyltransferase
»
Anti -Dnmt3a (DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA Methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA)
Anti -Dnmt3a (DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA Methyltransferase
HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B |
|
| DNMT3A is a 130kD member of the C5-methlytransferase family of molecules. It is expressed both during development and in virtually all adult tissues except for small intestine. DNMT3A is a nuclear protein that is found associated with heterochromatin. It possesses DNA methyltransferase activity, and acts in a complex with DNMT3L in embryonic stem cells, forming a (3A)2:(3L)2 heterotetramer. It also interacts with SETDB1 to form a transcriptional repressor. Human DNMT3A is 912aa in length. It contains one ProTrpTrpPro domain aa292-350 and one ADD-type zinc finger region aa494-586. There is one 100kD splice variant termed DNMT3a2 that contains an alternative start site at Met224, and preferentially interacts with euchromatin. Other potential splice variants show a 16aa substitution for aa151-912, and a 24aa substitution for aa1-213. Over aa353-486, human DNMT3A shares 96% aa identity with mouse DNMT3A. | | | Catalog # | D3931-70U | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2B | | Clone No | 12K80 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml after reconstitution | | Form | Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml. | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Recombinant corresponding to aa353-486 from human DNMT3A expressed in E.coli. | | Specificity | Recognizes human DNMT3A. Dos not react with recombinant human DNMT1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
