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You are here:Home » Antibodies » Abs to Enzymes, Methyltransferase » Anti -DNA Methyltransferase 3B (Dnmt 3B)

Anti -DNA Methyltransferase 3B (Dnmt 3B)


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Clone Host Grade Applications
Monoclonal Mouse Purified B IP IH IC IF
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
Catalog #D3931-73A
ApplicationsSuitable for use in Western blot, Immunoprecipitation, Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western blot: 2-4ug/ml. A band at 110kD is only detected in 293 cells transfected with mouse Dnmt3b cDNA.
Immunoprecipitation: 1-2ug/ml.
Immunofluorescence/Immunocytochemistry: 1-2ug/ml.
Immunohistochemistry: Paraffin sections.
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlNIH 3T3 cell lysate.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No4H84
FormSupplied as a liquid in PBS, 0.05%BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenBacteria expressed HIS-tag recombinant mouse Dnmt3b.
SpecificityRecognizes mouse DNA Methyltransferase 3B. Species Crossreactivity: Human. Does not react with Dnmt3a.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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