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Anti -DNA Methyltransferase 3L (Dnmt 3L, DNA Cytosine 5’ Methyltransferase 3 Like Protein)
Anti -DNA Methyltransferase 3L (Dnmt 3L, DNA Cytosine 5’ Methyltransferase 3 Like Protein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IP |
|
| DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have | | | demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. | | | Catalog # | D3931-76 | | Applications | Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 1:1000-1:3000 | | Western Blot: 1:1500. Detects human DNMT3L by Western Blot after Immunoprecipitation. Transfection of U2OS cells | | (10cm dish) was accomplished using 3ug of GAL4-DNMT3L. Protein extraction using IPH at 150mM. Does not perform well when applied directly for Western Blot. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50% ) glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | A synthetic peptide corresponding to aa 152-164, GLLQRRRKWRSQL of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases. | | Specificity | Recognizes Human DNMT3L MW | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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