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You are here:Home » Antibodies » Antibodies-Enzymes, Methyltransferase » Anti -DNA Methyltransferase 3L (Dnmt 3L, DNA Cytosine 5 Methyltransferase 3 Like Protein)

Anti -DNA Methyltransferase 3L (Dnmt 3L, DNA Cytosine 5 Methyltransferase 3 Like Protein)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP
DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have
demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
Catalog #D3931-76
ApplicationsSuitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:1000-1:3000
Western Blot: 1:1500. Detects human DNMT3L by Western Blot after Immunoprecipitation. Transfection of U2OS cells
(10cm dish) was accomplished using 3ug of GAL4-DNMT3L. Protein extraction using IPH at 150mM. Does not perform well when applied directly for Western Blot.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50% ) glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide corresponding to aa 152-164, GLLQRRRKWRSQL of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases.
SpecificityRecognizes Human DNMT3L MW
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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