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You are here:Home » Cloning » Cloning-Vectors » DNA Plasmid pBR322 E. coli RLM430

DNA Plasmid pBR322 E. coli RLM430

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Specifications

pBR322 is an E. coli plasmid vector bearing two antibiotic resistance genes: tetracycline and ampicillin. The conventional numbering of nucleotides begins with the first T in the unique EcoRI site and the numbering continues through the tetracycline gene. The peptide coding region of the tetracycline resistance gene ranges from nucleotide 86 to 1276 and the ampicillin resistance gene ranges from nucleotide 3293 to 4153. pBR322 is a circular double stranded DNA containing 4361 base pairs and having a molecular weight of 2.88X10e6 daltons.
Catalog #D3932
Source
pBR322 DNA is isolated from E.coli (dam+, dcm+) and purified by ultracentrifugation twice through a cesium chloride gradient in the presence of ethidium bromide. The molecule is a double-stranded circle, 4361 base pairs in length.
GenBank/EMBL accession numbers
J01749, K00005, L08654, M10282, M10283, M10286, M10356, M10784, M10785, M10786, M33694, V01119.
Form
Supplied in a solution of 10mM Tris HCl, pH 8.0, 1mM EDTA
Concentration
~0.5mg/ml
Quality Control
90% is in the supercoiled form.
Purity (A260/A280)1.8
Purified using a cesium chloride and ethidium bromide equilibrium gradient.
Equivalence50ug DNA products are equivalent to one A260 unit.
Storage & Handling
Store at -20°C. Once thawed, keep at 2-8°C. During prolonged storage the supercoiled form may be slowly converted into the relaxed circular form.


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