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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -DNA Polymerase, beta

Anti -DNA Polymerase, beta

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Reactive oxygen species (H2O2·, OH·, O2, HOCL) generated during normal cellular activities or by ionizing radiation and by many oxidizing/alkylating agents can induce a wide variety of lesions in DNA ranging from oxidized bases (8-oxoguanine and thymine glycol), base loss and DNA strand breaks with modified ends such as 3'-phosphate and 3'-phopshoglycolate. Unrepaired damaged/modified DNA has been implicated in aging and cancer.
Catalog #D3935-12A
Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed. DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MMNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells. DNA polymerase beta comprises an amino-terminal 8-kD domain and a carboxy-terminal 31kD domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-5ug/ml. The antibody has detected ~39kD band from HeLa and other cells and tissues.
ELISA: 1:10,000-50,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No3H14
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
PurityPurified by protein A/G affinity chromatography.
ImmunogenPurified Rat DNA Pol beta (~39kD)
SpecificityRecognizes rat DNA Polymerase, beta. Species Crossreactivity: Mouse, human, hamster and frog.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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