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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -DNA Polymerase, beta

Anti -DNA Polymerase, beta

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B
During base excision repair (BER) DNA polymerase b (Pol b ) is recruited by APE1. Pol b has two distinct domains, the larger is the polymerase domain itself, whereas a small basic N-terminal domain contains an AP lyase activity that excises the abasic sugar-phosphate residue at the strand break. Pol b also interacts with XRCC1, DNA ligase III and poly(ADP-ribose) polymerase in the repair of DNA single strand breaks. Pol b plays a role in both BER pathways, "single-nucleotide" and "long patch". It is the most inaccurate of the six DNA polymerases found in mammalian cells. Overexpression of Pol , as found in some human tumors, could confer an increase in spontaneous mutagenesis. It has been further suggested that Pol b does not excise 5'-oxidized AP sites induced by reactive oxygen species (ROS).
Catalog #D3935-12D
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid, 0.05% sodium azide.
PuritySerum
ImmunogenRecombinant human Pol b.
SpecificityRecognizes human DNA Polymerase-beta. Detects a band of ~45kD by Western Blot. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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