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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -DNA Polymerase, beta (BSA & Azide Free)

Anti -DNA Polymerase, beta (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
DNA polymerase comprises an amino-terminal 8kD domain and a carboxyterminal 31kD domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase b, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-Ní-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase b fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
Catalog #D3935-12X
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 2-4ug/ml for 2 hrs at RT
Immunohistochemistry (Formalin/paraffin): 20 ug/ml for 60min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control HeLa cells. Testis
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3F146
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See D3935-12
PurityPurified by Protein A affinity chromatography.
ImmunogenDNA polymerase beta protein. Cellular Localization: Nuclear.
SpecificityRecognizes DNA Polymerase, beta. MW
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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