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You are here:Home » Antibodies » Antibodies-Enzymes, Polymerase » Anti -DNA Polymerase, epsilon (Polepsilon, CHRAC 17, Chromatin Accessibility Complex 17, DNA Polymerase Epsilon p17 Subunit, DNA Polymerase Epsilon Subunit 3, 17, YBL1)

Anti -DNA Polymerase, epsilon (Polepsilon, CHRAC 17, Chromatin Accessibility Complex 17,
DNA Polymerase Epsilon p17 Subunit, DNA Polymerase Epsilon Subunit 3, 17, YBL1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
Pol e is present in proliferating cells and is implicated in repair of UV-damaged DNA. It is found to be a component of a recombination complex that is able to repair deletions and double-strand breaks. It is not known whether pol e* can
replace pol e in these processes.
Catalog #D3935-25
ApplicationsSuitable for use in Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionsImmunoprecipitation (Native and Denatured): 2ug/mg protein lysate.
Western Blot: 2-4ug/ml for 2hours at RT.
Optimal dilutions to be determined by the researcher.
Recommended Positve Control293 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.250
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See D3935-25X.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified recombinant protein containing sequence corresponding to residues 1-176 of human Pol e. Cellular Localization: Nuclear.
SpecificityRecognizes human DNA polymerase e (Pol e) at ~ 200kD. Also recognizes a 140kD form of Pole , known as Pol e*, a truncated from of pole that has lost its C-terminal half. Species Crossreactivity: monkey, bovine and mouse, S. cerevisiae both Pol e and Pole * . D. melanogaster, T. ni. insect cells, X. laevis and chicken, Pole only
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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