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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -DNA Polymerase, epsilon

Anti -DNA Polymerase, epsilon

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
DNA polymerase e from HeLa cells differs from yeast pol e in that HeLa pol e contains two subunits of 220 and 55kD, whereas yeast pol e has one large subunit and three smaller subunits. Previous studies of template specificity of HeLa pol e, which appears to be required for long patch DNA repair in permeabilized cells, indicate pol e prefers long stretches of single-stranded regions in poly(dA)-oligo(dT) as template. HeLa pol a and pol d prefer
activated DNA and alternating poly(dA-dT) as templates, respectively. Pol e retains its processivity in the absence of proliferating cell nuclear antigen (PCNA), unlike pol d. PCNA can also interact with Pol e on linear DNA templates, even in the absence of other auxiliary factors (replication factor C, replication factor A), and thereby stimulate its primer recognition and DNA synthesis.
Catalog #D3935-28
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3C133
HostMouse
SourceHuman
ConcentrationNot determined
PurityPurified by Protein G affinity chromatography.
ImmunogenMono S FPLC column purified HeLa cell polymerase epsilon.
SpecificitySpecific to the human DNA pol epsilon and does not recognize DNA pol alpha, beta or delta. Species crossreactivity: Can be used for detection of pol epsilon in human, mouse, monkey, mink and hamster.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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