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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -DNA Polymerase, eta (PolH, XPV, Xeroderma Pigmentosum Variant Type Protein, RAD30, RAD30A)

Anti -DNA Polymerase, eta (PolH, XPV, Xeroderma Pigmentosum Variant Type Protein, RAD30,
RAD30A)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
DNA polymerase eta is specifically involved in DNA repair. It plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. It also plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. It targets POLI to replication foci.
Catalog #D3935-33J
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:2000-1:10,000
Immunoprecipitation: 2-5ug/mg lysate
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in Tris-buffered saline, 0.1% BSA, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide composed of amino acids 690-713 of human Polymerase (DNA directed), eta using the numbering given in entry NP_006493.1 (GeneID 5429).
SpecificityRecognizes human DNA Polymerase, eta.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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