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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -DNA Polymerase, gamma

Anti -DNA Polymerase, gamma

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Mitochondria produce cellular ATP through oxidative phosphorylation. Mitochondrial DNA encodes several proteins necessary for oxidative phosphorylation, whereas many other genes are nuclear. DNA Pol gamma (POLG), the only DNA polymerase present in mitochondria, has been implicated in base excision repair process. The catalytic subunit of POLG catalyzes the release of dRP (5' terminal deoxyribose phosphate) from incised apurinic/apyrimidinic sites to produce a substrate for DNA ligase. POLG deficiency has been associated with mtDNA depletion and Alper's syndrome. Low selectivity of mtDNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV.
Catalog #D3935-35A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-5ug/ml. The antibody recognizes >140kD DNA Pol g from HeLa and other cells and tissues.
ELISA: 1:5000-25,000 using 50-100ng control antigen/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H16
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in 10 mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified human recombinant DNA Pol gamma aa26-276
SpecificityRecognizes human POLG. No crossreactivity is seen with DNA Pol a, b or d. Antibody crossreactivity in various other species has not been studied
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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