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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -DNA Polymerase, gamma (DNA Polymerase Subunit gamma-1, Mitochondrial DNA Polymerase Catalytic Subunit, PolG-alpha, POLG, MDP1, POLG1, POLGA)

Anti -DNA Polymerase, gamma (DNA Polymerase Subunit gamma-1, Mitochondrial DNA Polymerase
Catalytic Subunit, PolG-alpha, POLG, MDP1, POLG1, POLGA)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
As the only DNA polymerase (pol) present in mitochondria, DNA polymerase gamma (POLG) is necessarily implicated in base excision repair processes to correct oxidative damage to the mitochondrial genome. Therefore, the ability of the catalytic subunit of human POLG has been tested to participate in uracil-provoked base excision repair reconstituted in vitro with purified components. Subsequent to actions of uracil-DNA glycosylase and apurinic/apyrimidinic endonuclease, human POLG is able to fill a single nucleotide gap in the presence of a 5' terminal deoxyribose phosphate (dRP) flap. The catalytic subunit of human pol gamma catalyzes release of the dRP residue from incised apurinic/apyrimidinic sites to produce a substrate for DNA ligase. Faulty replication of the human mitochondrial genome is thought to be the cause of many diseases. The low selectivity of the mitochondrial DNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV.
Catalog #D3935-37A
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 5-10ug/ml for 2h,at RT
Immunoprecipitation: 10ug/mg protein lysate
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified native human recombinant (aa714-1061) DNA polymerase gamma.
SpecificityRecognizes human DNA Polymerase gamma. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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