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Specifications

Clone Host Grade Applications Polyclonal Human Purified E IF

Catalog #	D4015
Applications	Suitable for use in Immunofluorescence and ELISA. Other applications have not been tested.
Recommended Dilution	ELISA: 1:100
	Optimal dilutions to be determined by the researcher.
Biological Activity	Shows anti-dsDNA activity only in ELISA.
Storage and Stability	Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Human
Source	Human
Concentration	Not Determined
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
Purity	Fractionated from delipidated, defibrinated and precipitated human serum.
Immunogen	DNA, ss. Derived from donor human serum.
Specificity	Recognizes human ds-DNA. Exhibits strong positive reaction in an anti-dsDNA ELISA system. Shows no reactivity at a dilution of 1:100 when tested against purified Ro/SS-A, La/SS-B, Sm, nRNP, Scl-70, ssDNA and Jo-1 antigens in an ELISA system designed to detect IgG antibody. Negative for ssDNA in RIA.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.