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Specifications

Clone Host Grade Applications Polyclonal Human Purified E

Anti-ssDNA (Single-stranded DNA) allows the important discrimination between apoptosis and necrosis. In this respect it can be regarded as a unique research tool superior to TUNEL staining.
Catalog #	D4015-05
Applications	Suitable for use in ELISA and Farr Assay. Other applications have not been tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Biological Activity	Farr Assay: Demonstrates anti-ssDNA activity.
	Note: 24% (normal <13%) of the radio-iodinated DNA precipitates with this antibody..
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Human
Source	Human
Concentration	Not Determined
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 0.02% sodium azide. Reconstitute in 2ml sterile ddH2O.
Purity	Purified by fractionation of delipidated and defibrinated donor human serum.
Immunogen	Derived from donor human serum.
Specificity	Displays only Anti-ssDNA activity when tested by ELISA. Undiluted antibody shows a strong Scl-70 precipitin band. Specificity verified by a precipitin line of identity with the CDC-AF reference serum and by ELISA procedures using both native DNA and ssDNA. Does not crossreact at a dilution of 1:100 when tested against purified Ro/SS-A, La/SS-B, Sm, Scl-70, nRNP, dsDNA, or Jo-1 antigens in an ELISA system designed to detect IgG
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.