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You are here:Home » Antibodies » Abs to Nucleic Acids » Anti -DNA, ss

Anti -DNA, ss

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Specifications

Clone Host Grade Applications
Polyclonal Human Purified E
Anti-ssDNA (Single-stranded DNA) allows the important discrimination between apoptosis and necrosis. In this respect it can be regarded as a unique research tool superior to TUNEL staining.
Catalog #D4015-05
ApplicationsSuitable for use in ELISA and Farr Assay. Other applications have not been tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Biological ActivityFarr Assay: Demonstrates anti-ssDNA activity.
Note: 24% (normal <13%) of the radio-iodinated DNA precipitates with this antibody.
Autoantibody ActivityAnti-ssDNA Activity by ELISA: Current lot value: 397IU/ml
Interpretation of Results: <16 EU/ml: Negative; 16-20EU/ml: Equivocal; Positive; >20 EU/ml:
SSA: 129 EU/ml
SSB, SCL, Sm: 0 EU/ml
Sm/RNP, Jo-1: 1 EU/ml
dsDNA: 32 EU/ml
Anti-ssDNA Activity by ELISA As reported (lot-specific). Current lot value: 397U/ml
Interpretation of Results: <68.6 U/ml: Negative; >68.6U/ml: Positive
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostHuman
SourceHuman
ConcentrationNot Determined
FormSupplied as a lyophilized powder from PBS, pH 7.2, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
PurityPurified by fractionation of delipidated and defibrinated donor human serum.
ImmunogenDerived from donor human serum.
SpecificityDisplays only Anti-ssDNA activity when tested by ELISA. Undiluted antibody shows a strong Scl-70 precipitin band. Specificity verified by a precipitin line of identity with the CDC-AF reference serum and by ELISA procedures using both native DNA and ssDNA. Does not crossreact at a dilution of 1:100 when tested against purified Ro/SS-A, La/SS-B, Sm, Scl-70, nRNP, dsDNA, or Jo-1 antigens in an ELISA system designed to detect IgG
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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