| HDJ 2 is a novel human homolog of E. coli’s DNAJ protein from heat shock protein 40 family. HSP40 is involved in chaperonic protein folding. HDJ 2 is cytoplasmic and a nuclear membrane associated protein. Upon heat shock it migrates and becomes associated with the Golgi complex, nucleolus and nuclear membrane. HDJ 2 has a prenylated C-terminus and is not inducible upon heat shock. |
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| Catalog # | D4015-09A |
| Applications | Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Immunoprecipitation: 2ug/mg protein lysate (native and denatured). Use Protein G. |
| Western Blot: 0.5-1ug/ml for 2hr at RT. |
| Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Staining of formalin/paraffin tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | MAD109, Raji cells. Tonsil. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3F200 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See D4015-09AX. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | N-terminal puri ed recombinant fragment (aa1-179) of HDJ 2 fusion protein. Cellular Localization |
| Specificity | Recognizes HDJ 2 (DNAJ). Highly specific to human HDJ 2 and shows no crossreactivity with HDJ 1. Associates with a 53kD protein of unknown identity. Species Crossreactivity: monkey, porcine, rabbit, mouse, rat, hamster and bacteria. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
