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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -DnaJ (HSP40, HDJ 2)

Anti -DnaJ (HSP40, HDJ 2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC IF
HDJ 2 is a novel human homolog of E. coliís DNAJ protein from heat shock protein 40 family. HSP40 is involved in chaperonic protein folding. HDJ 2 is cytoplasmic and a nuclear membrane associated protein. Upon heat shock it migrates and becomes associated with the Golgi complex, nucleolus and nuclear membrane. HDJ 2 has a prenylated C-terminus and is not inducible upon heat shock.
Catalog #D4015-09A
ApplicationsSuitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunoprecipitation: 2ug/mg protein lysate (native and denatured). Use Protein G.
Western Blot: 0.5-1ug/ml for 2hr at RT.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Staining of formalin/paraffin tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlMAD109, Raji cells. Tonsil.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No3F200
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See D4015-09AX.
PurityPurified by Protein G affinity chromatography.
ImmunogenN-terminal puri ed recombinant fragment (aa1-179) of HDJ 2 fusion protein. Cellular Localization
SpecificityRecognizes HDJ 2 (DNAJ). Highly specific to human HDJ 2 and shows no crossreactivity with HDJ 1. Associates with a 53kD protein of unknown identity. Species Crossreactivity: monkey, porcine, rabbit, mouse, rat, hamster and bacteria.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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