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You are here:Home » Antibodies » Antibodies-Heat Shock Proteins » Anti -DnaJ (HSP40, HDJ 2) (BSA & Azide Free)

Anti -DnaJ (HSP40, HDJ 2) (BSA & Azide Free)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC IF
Novel human homolog of E. coliís DnaJ protein from heat shock protein 40 family. HSP40 is involved in chaperonic protein folding. HDJ 2 is cytoplasmic and nuclear membrane associated protein; upon heat shock it migrates and becomes associated with the Golgi complex, nucleolus and nuclear membrane. HDJ 2 has a prenylated C-terminus and is not inducible upon heat shock.
Catalog #D4015-09AX
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.5-1ug/ml for 2 hours ar RT.
Immunoprecipitation (Native and denatured): 2ug/mg protein lysate. Use Protein G.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlMAD109, Raji cells, Tonsil
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No3F200
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See D4015-09A.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant fragment corresponding to aa1-179 at the N-terminal of HDJ-2 fusion protein. MW of Antigen
SpecificityRecognizes human HDJ 2 (DnaJ). No crossreactivity with HDJ 1. Associates with a 53kD protein of unknown identity.
Species CrossreactivityMonkey, porcine, rabbit, mouse, rat, hamster and bacteria.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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