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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -DnaJ (HSP40, HDJ 2) (BSA & Azide Free)

Anti -DnaJ (HSP40, HDJ 2) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC IF
Novel human homolog of E. coliís DnaJ protein from heat shock protein 40 family. HSP40 is involved in chaperonic protein folding. HDJ 2 is cytoplasmic and nuclear membrane associated protein; upon heat shock it migrates and becomes associated with the Golgi complex, nucleolus and nuclear membrane. HDJ 2 has a prenylated C-terminus and is not inducible upon heat shock.
Catalog #D4015-09AX
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-1ug/ml for 2 hrs ar RT.
Immunoprecipitation (Native and denatured): 2ug/mg protein lysate. Use Protein G.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlMAD109, Raji cells. Tonsil.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3F200
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See D4015-09A.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant fragment (aa1-179), N-terminal of HDJ-2 fusion protein. MW of Antigen
SpecificityRecognizes human HDJ 2 (DnaJ). No crossreactivity with HDJ 1. Associates with a 53kD protein of unknown identity. Species Crossreactivity: Monkey, porcine, rabbit, mouse, rat, hamster and bacteria.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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