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You are here:Home » Antibodies » Abs to Enzymes, Deoxyribonuclease » Anti -Deoxyribonuclease II (DNase II) (CT)

Anti -Deoxyribonuclease II (DNase II) (CT)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified B
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus (1). DNase II causes both chromatin condensation and DNA fragmentation (1). The genes encoding human (2-4), porcine (4), and murine (5) DNase II have been cloned. The DNase II gene encodes a 40kD proenzyme. The mature enzyme consists of two non-identical subunits, the 32 and 12kD (b) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation (3). DNase II is ubiquitously expressed in human tissues.
Catalog #D4020-15
This polyclonal antibody can be used for detection of DNase II expression by Western blot at 1500 to 1:1000 dilution. Tissue lysate of human spleen can be used as positive control and an approximately 32kD (a chain) band can be detected. It doesnít recognize the 12kD b chain.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration0.5mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityImmunoaffinity purified IgG
ImmunogenPeptide corresponding to amino acids 347 to 360 of human DNase II precursor (2-4).
SpecificityRecognizes human DNase II (CT), ~32kD band can be detected (alpha chain). Does not recognize the 12kD beta chain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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