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You are here:Home » Antibodies » Abs to DEAH Box Proteins » Anti -DHX29 (DEAH (Asp-Glu-Ala-His) Box 29, DDX29, FLJ21492, Nucleic acid helicase DDX)

Anti -DHX29 (DEAH (Asp-Glu-Ala-His) Box 29, DDX29, FLJ21492, Nucleic acid helicase DDX)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
DHX29 is an ATP-dependent RNA helicase that belongs to the DEAD-box helicase family (DEAH subfamily). DHX29 contains one central helicase and one helicase at the carboxy-terminal domain (1). Its function has not been fully established but DHX29 was recently shown to facilitate translation initiation on mRNAs with structured 5 untranslated regions (2). DHX29 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. Hydrolysis of nucleotide triphosphates by DHX29 is strongly stimulated by 43S complexes and is required for DHX29 activity in promoting 48S complex formation (2).
Catalog #D6510-40C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA and 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide derived from a sequence around Gly63 of human DHX29 protein (KLH-coupled).
SpecificityRecognizes human DHX29 (G63). Species crossreactivity: mouse, rat, monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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