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2', 3'-Dideoxynucleotide-5'-triphosphate Set (ddNTP) (20mM, 4x1umole, ddATP, ddCTP, ddGTP, ddTTP)
2', 3'-Dideoxynucleotide-5'-triphosphate Set (ddNTP) (20mM, 4x1umole, ddATP, ddCTP, ddGTP, ddTTP)
Pricing
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| 2', 3'-ddNTP is a set of 4 synthetic triphosphates lacking of 2'- and 3'- hydroxyl groups, that results in DNA chain termination. Suitable for sequencing DNA by the methods of Sanger and cycle sequence. | | | Catalog # | D7880-26 | | Applications | Suitable for sequencing DNA by the methods of Sanger and cycle sequence. | | Concentration | 4x1umol (20mM) | | Quality Control | | | Functionally tested in cycle sequence with Taq DNA Polymerase™. | | | Purity (HPLC) | 95% | | | Lambda Max | | | ddATP | 261 | | ddCTP | 281 | | ddGTP | 253 | | ddTTP | 267 | | | Extinction Coefficient | | | ddATP | 15.2x10e3 | | ddCTP | 9.3x10e3 | | ddGTP | 13.6x10e3 | | ddTTP | 9.6x10e3 | | | A250/A260 | | | ddATP | 0.74 ± 0.03 | | ddCTP | 0.41 ± 0.03 | | ddGTP | 0.15 ± 0.03 | | ddTTP | 0.62 ± 0.03 | | | A280/A260 | | | ddATP | 0.17 ± 0.03 | | ddCTP | 2.41 ± 0.03 | | ddGTP | 0.67 ± 0.03 | | ddTTP | 0.81 ± 0.03 | | | Storage and Stability | | | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | | ™The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffman-La Roche. |
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