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You are here:Home » Biochemicals » Biochem-Nucleotides » 2', 3'-Dideoxynucleotide-5'-triphosphate Set (ddNTP) (20mM, 4x1umole, ddATP, ddCTP, ddGTP, ddTTP)

2', 3'-Dideoxynucleotide-5'-triphosphate Set (ddNTP) (20mM, 4x1umole, ddATP, ddCTP, ddGTP,
ddTTP)

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Specifications

2', 3'-ddNTP is a set of 4 synthetic triphosphates lacking of 2'- and 3'- hydroxyl groups, that results in DNA chain termination. Suitable for sequencing DNA by the methods of Sanger and cycle sequence.
Catalog #D7880-26
ApplicationsSuitable for sequencing DNA by the methods of Sanger and cycle sequence.
Concentration 4x1umol (20mM)
Quality Control
Functionally tested in cycle sequence with Taq DNA Polymerase™.
Purity (HPLC)95%
Lambda Max
ddATP261
ddCTP281
ddGTP253
ddTTP267
Extinction Coefficient
ddATP15.2x10e3
ddCTP9.3x10e3
ddGTP13.6x10e3
ddTTP9.6x10e3
A250/A260
ddATP0.74 ± 0.03
ddCTP0.41 ± 0.03
ddGTP0.15 ± 0.03
ddTTP0.62 ± 0.03
A280/A260
ddATP0.17 ± 0.03
ddCTP2.41 ± 0.03
ddGTP0.67 ± 0.03
ddTTP0.81 ± 0.03
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
™The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffman-La Roche.


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