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Anti -Dgcr8 (Microprocessor Complex Subunit DGCR8, DiGeorge Syndrome Critical Region 8 Homolog, Gy1)
Anti -Dgcr8 (Microprocessor Complex Subunit DGCR8, DiGeorge Syndrome Critical Region 8
Homolog, Gy1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B |
|
| Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal. | | | Catalog # | D8010-16D | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 1:64,000 | | Western Blot: 0.1-0.3ug/ml, observed in nuclear lysates of cell line NIH3T3 on ~90kD bands | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Goat | | Source | Mouse | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to C-PDFVKQTSEEKPKD, from mouse DGCR8, at the internal region of the protein (NP_073557.3, NP_001177255.1). | | Specificity | Recognizes mouse DGCR8. Species sequence homology: Bovine, canine, human, porcine and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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