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You are here:Home » Antibodies » Abs to Disease Markers » Anti -Dgcr8 (Microprocessor Complex Subunit DGCR8, DiGeorge Syndrome Critical Region 8 Homolog, Gy1)

Anti -Dgcr8 (Microprocessor Complex Subunit DGCR8, DiGeorge Syndrome Critical Region 8
Homolog, Gy1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal.
Catalog #D8010-16D
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:64,000
Western Blot: 0.1-0.3ug/ml, observed in nuclear lysates of cell line NIH3T3 on ~90kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-PDFVKQTSEEKPKD, from mouse DGCR8, at the internal region of the protein (NP_073557.3, NP_001177255.1).
SpecificityRecognizes mouse DGCR8. Species sequence homology: Bovine, canine, human, porcine and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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