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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E IH

Digoxigenin is considered a hapten, a small molecule with high immunigenicity used in many molecular biology applications. Other popular haptens include DNP (dinitrophenol), biotin and fluorescein. In general, antibodies generated against haptens have higher affinities for their targets than other antibodies, so haptens are conjugated to other biological molecules as all-purpose immuno-tags. This is because anti-hapten antibodies have high affinities and are readily available, while antibodies may not be commercially available for exotic biological targets.
Digoxigenin is a standard immunohistochemical marker for in situ hybridization. In this case it is conjugated to a single species of RNA nucleotide triphosphate (typically Uridine), which is then incorporated into RNA (a "riboprobe") as it is synthesized by the cellular machinery. Later, the Digoxigenin ("Dig") markers are detected with anti-digoxigenin antibodies, which may be labeled with dyes or enzymes (see ELISA) for visualization and detection. In a similar manner, Digoxigenin may be conjugated to sugars to study glycosylation events in biological systems.
Catalog #	D8029-50
Applications	Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Immunohistochemistry (Paraffin): 1:200-1:2000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	8J1299
Host	Mouse
Concentration	Not determined
Form	Supplied as a lyophilized powder. No preservative added. Reconstitute with 200ul sterile ddH2O.
Purity	Ascites
Immunogen	Free and bound Digoxigenin.
Specificity	Recognizes free and bound Digoxigenin.
Species Crossreactivity	All species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.