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You are here:Home » Antibodies » Antibodies-Epitope Tags » Anti -Digoxigenin (DIG)

Anti -Digoxigenin (DIG)


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Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH
Digoxigenin is considered a hapten, a small molecule with high immunigenicity used in many molecular biology applications. Other popular haptens include DNP (dinitrophenol), biotin and fluorescein. In general, antibodies generated against haptens have higher affinities for their targets than other antibodies, so haptens are conjugated to other biological molecules as all-purpose immuno-tags. This is because anti-hapten antibodies have high affinities and are readily available, while antibodies may not be commercially available for exotic biological targets. Digoxigenin is a standard immunohistochemical marker for in situ hybridization. In this case it is conjugated to a single species of RNA nucleotide triphosphate (typically Uridine), which is then incorporated into RNA (a "riboprobe") as it is synthesized by the cellular machinery. Later, the Digoxigenin ("Dig") markers are detected with anti-digoxigenin antibodies, which may be labeled with dyes or enzymes (see ELISA) for visualization and detection. In a similar manner, Digoxigenin may be conjugated to sugars to study glycosylation events in biological systems.
Catalog #D8029-50C
ApplicationsSuitable for use in ELISA, Immunohistochemistry, and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:100-1:5000
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenDigoxin-3-CMO (KLH)
SpecificityRecognizes Digoxigenin. Crossreactivity: Digoxin, Digitioxin. Negligible reactivity has been observed with Ouabain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesLanadigenin

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