Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Assay Detection » Anti -Digoxigenin

Anti -Digoxigenin

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Sheep Purified E
Digoxigenin is considered a hapten, a small molecule with high immunogenicity used in many molecular biology applications. Other popular haptens include DNP (dinitrophenol), biotin, and fluorescein. In general, antibodies generated against haptens have higher affinities for their targets than other antibodies, so haptens are conjugated to other biological molecules as all-purpose immuno-tags. This is because anti-hapten antibodies have high affinities and are readily available, while antibodies may not be commercially available for exotic biological targets. Digoxigenin is a standard immunohisto- chemical marker for in situ hybridization. In this case it is conjugated to a single species of RNA nucleotide triphosphate (typically Uridine), which is then incorporated into RNA (a "riboprobe") as it is synthesized by the cellular machinery. Later, the digoxigenin ("Dig") markers are detected with anti-digoxigenin antibodies, which may be labeled with dyes or enzymes (see ELISA) for visualization and detection. In a similar manner, digoxigenin may be conjugated to sugars to study glycosylation events in biological systems.
Catalog #D8029-50J
ApplicationsSuitable for use in ELISA and in the development of immunoassays or immunoaffinity purification columns. Other applications not tested.
Recommended DilutionELISA: 0.625ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostSheep
Concentration~1mg/ml
FormSupplied as a liquid in 20mM phosphate, 150mM sodium chloride, pH 7.2, 0.09% sodium azide.
PurityPurified
ImmunogenDigoxigenin (BTG)
SpecificityRecognizes digoxigenin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links