Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:3200 dilution detected 0.312ug/ml HPLC purified standard DNP. Optimal dilutions to be determined by the researcher.
AA Sequence
Glu-Val-Lys-Tyr-Asp-Pro-Cys-Phe-Gly-His-Lys-Ile-Asp-Arg-Ile-Asn-His-Val-Ser-Asn-Leu-Gly-Cys-Pro-Ser-Leu-Arg-Asp-Pro-Arg-Pro- Asn-Ala-Pro-Ser-Thr-Ser-Ala
Disulfide Bridge: Cys7 - Cys23
Storage and Stability
Lyophilized and reconstituted products are stable for 12 /PBSvmonths after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to green mamba DNP.
Form
Supplied as a powder lyophilized from a solution containing PBS. Reconstitute with 220ul sterile ddH2O and 180ul PBS.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes green mamba DNP. Exhibits 0% cross-reactivity with: alpha-ANP 1-28 (human, canine, porcine, bovine), human Prepro-ANF 26-55, CNP-22 (human, porcine, rat), human BNP-32, Sarafotoxin S6a, Sarafotoxin S6b, Sarafotoxin S6c, Endothelin-1 (human, rat, porcine, mouse, canine, bovine).