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You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -Dipeptidyl Peptidase 10 (DPP10)

Anti -Dipeptidyl Peptidase 10 (DPP10)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B
DPP10 (dipeptidyl peptidase 10; also DPPY, DPRP3 and DPL2) is a 91-97kD member of the peptidase S9B family. It is a type II transmembrane glycoprotein principally expressed in neurons and T cells. Although it is a peptidase member, it has no catalytic activity. Instead, it assists in the membrane trafficking and functioning of Kv4 K+ channels. Human DPP10 is 796 amino acids (aa) in length. It contains a 34 aa cytoplasmic N-terminus and a 741 aa extracellular domain (ECD) (aa 56-796). Three potential isoforms exist, all involving aa substitutions limited to the first 20 amino acids. The ECD of human DPP10 shares 89% aa sequence identity with mouse DPP10 ECD.
Catalog #D8065-33
ApplicationsWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human DPP10. The detection limit for rhDPP10 is approximately 2 ng/lane under non-reducing and reducing conditions.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human DPP10. The detection limit for rhDPP10 is approximately 0.3 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
PurityPurified by human DPP10 affinity chromatography.
ImmunogenSf21-derived DPP10 isoform 1 extracellular domain
SpecificitySelected for its ability to recognize human DPP10 in direct ELISAs and Western Blots. In these formats, this antibody shows less than 5% crossreactivity with rhDPP6 and rhDPP4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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