You are here:Home
»
Antibodies
»
Abs to Enzymes, Methyltransferase
»
Anti -DMAP1 (CT) (DNA Methyltransferase 1-Associated Protein 1, DNMAP1, DNMTAP1, DNMT1-Associated Protein 1, MAT1-Mediated Transcriptional Repressor)
Anti -DMAP1 (CT) (DNA Methyltransferase 1-Associated Protein 1, DNMAP1, DNMTAP1,
DNMT1-Associated Protein 1, MAT1-Mediated Transcriptional Repressor)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IH IC |
|
| DNA methylation can help to regulate transcriptional silencing via repressive transcription complexes, which include methyl-CpG-binding domain proteins and histone deacetylases (HDACs). DNMT1, the core enzyme for mammalian DNA methylation, can also establish a repressive transcription complex consisting of DNMT1, HDAC2, and a third protein, termed DMAP1 for 'DNMT1-associated protein.' The 467-amino acid DMAP1 protein shares approximately 98% amino acid sequence homology with the mouse protein. DMAP1 interacts directly with the N-terminal region of DNMT1, and DMAP1 can repress transcription independently of histone deacetylase activity. DNMT1, HDAC2, and DMAP1 form a complex in vivo, and DMAP1 can interact directly with the transcriptional corepressor TSG101. The DNMT1-DMAP1 exists throughout the S phase; HDAC2 joins DNMT1 and DMAP1 only during late S phase. This provides a regulated means to deacetylate heterochromatin following replication. | | | Catalog # | D8073-02D | | Applications | Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. | | Recommended Dilution | ELISA: 1:1000 | | Western Blot: 1:100-1:500 | | Immunohistochemistry (paraffin): 1:50-100 | | Immunocytochemistry: 1:500 | | Optimal dilutions to be determined by the researcher. | | Positive Control | HL60 cell lysate | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | ~0.25mg/ml | | Form | Supplied as a liquid in PBS, 0.09% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Synthetic peptide corresponding to the C-terminal region of DMAP1. Cellular Localization: Nucleus. | | Specificity | Recognizes human DMAP1. Species sequence homology: mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
