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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Dentin Matrix Protein 1 (DMP1)

Anti -Dentin Matrix Protein 1 (DMP1)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH
DMP-1 (dentin matrix protein 1) is an acidic, 100-160kD member of the phosphophoryn family of extracellular matrix proteins. Mouse DMP-1 is found both intra- and extracellularly, where phosphorylated DMP-1 participates in bone mineralization. Secreted mature mouse DMP-1 is a 487 amino acid (aa) proteoglycan. It contains multiple serine, glutamic acid, and aspartic acid residues, a GAG attachment site (aa71-72) and an RGD motif (aa350-352). Multiple cleavage sites exist between aa205-253, creating multiple fragments ~37kD and 57kD in size.
Catalog #D8075-51
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.
Recommended Dilutions:
Western Blot: 0.1-0.2ug/ml The detection limit is ~1ng/lane and 5ng/lane under non-reducing and reducing conditions, respectively.
Immunohistochemistry: 10ug/ml detects DMP1 in E15.5 mouse embryos. Fix cells with PBS, 4% paraformaldehyde for 20 minutes at RT and block with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. Incubate cells with D8075-51 for 3 hours at RT followed by donkey anti-sheep lgG at RT for 1 hour. Between each step, wash cells with PBS, 0.1% BSA.
Direct ELISA: 0.5-1.0ug/ml The detection limit is ~0.1ng/well.
Optimal dilutions to be determined by the researcher.
HybridomaNSO myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceMouse
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenRecombinant corresponding to aa17-503 from mouse DMP-1 expressed in NSO cells (AAI13754).
SpecificityRecognizes mouse DMP-1. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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