| DMP-1 (dentin matrix protein 1) is an acidic, 100-160kD member of the phosphophoryn family of extracellular matrix proteins. Mouse DMP-1 is found both intra- and extracellularly, where phosphorylated DMP-1 participates in bone mineralization. Secreted mature mouse DMP-1 is a 487 amino acid (aa) proteoglycan. It contains multiple serine, glutamic acid, and aspartic acid residues, a GAG attachment site (aa71-72) and an RGD motif (aa350-352). Multiple cleavage sites exist between aa205-253, creating multiple fragments ~37kD and 57kD in size. |
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| Catalog # | D8075-51 |
| Applications | Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested. |
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| Recommended Dilutions: |
| Western Blot: 0.1-0.2ug/ml The detection limit is ~1ng/lane and 5ng/lane under non-reducing and reducing conditions, respectively. |
| Immunohistochemistry: 10ug/ml detects DMP1 in E15.5 mouse embryos. Fix cells with PBS, 4% paraformaldehyde for 20 minutes at RT and block with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. Incubate cells with D8075-51 for 3 hours at RT followed by donkey anti-sheep lgG at RT for 1 hour. Between each step, wash cells with PBS, 0.1% BSA. |
| Direct ELISA: 0.5-1.0ug/ml The detection limit is ~0.1ng/well. |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | NSO myeloma cells with spleen cells from Balb/c mice. |
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| Storage and Stability: |
| Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | N/A |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Sheep |
| Source | Mouse |
| Concentration | ~0.2mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Recombinant corresponding to aa17-503 from mouse DMP-1 expressed in NSO cells (AAI13754). |
| Specificity | Recognizes mouse DMP-1. Species Crossreactivity |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
