| DM-GRASP (BEN, SC1) is an adhesion molecule that was originally described as a marker in the developing central and peripheral chicken nervous systems, where it was expressed by subsets of motor and sensory nervous during phases of axonogenesis, neurite elongation, axonal fasciculation, and synapse formation (2). DM-GRASP belongs to the CAM immunoglobulin superfamily of adhesion molecules, and exists as a cell surface glycoprotein of ~90-105kD (1). Structurally, DM-GRASP is related to MUC18, a human melanoma marker, but sequence identity between the two proteins is only ~25% (1). Homophilic GRASP-GRASP interactions between a growing neuron and a substrate appear to be an essential component of DM-GRASP’s neurite extension-stimulating activity (4). In chickens, levels of DM-GRASP expression on neurons are highest during the period of active neuronal extension, and disappear after they have reached their targets (5). A similar pattern has been observed during chick neuromuscular development; both motoneurons and their target, the myoblasts, express DM-GRASP during early embryonic development, then demonstrate restricted protein expression at neuromuscular |
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| contacts as soon as postsynaptic acetylcholine receptor clusters are observed (6). |
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| Applications: |
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| Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
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| Catalog # | D8075-65 |
| Recommended Dilution | Immunofluorescence (Rat): 1-5ug/ml |
| ELISA: 0.1-1.0ug/ml |
| Immunoprecipitation (Human, Rat): 5-10ug/test |
| Immunohistochemistry (Frozen) (Human, Rat): 0.5-5ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Positive Controls | Rat and human embryos (IHC), rat B49 cells (IF, IP), HCA-1-transfected COS-7 cells (IP). |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | N/A |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 6A66 |
| Host | Mouse |
| Source | Rat |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Rat neuronal cell line XKM |
| Specificity | Recognizes rat DM-GRASP at ~90-105kD. Species Crossreactivity: human |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
