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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant IH

Dog1 is a cell surface protein of unknown function selectively expressed in GIST. Among GIST cases with KIT mutations the Dog1 antibody identified 11% more cases than c-Kit antibody . Dog1 identifies vast majority of both c-Kit negative and PDGFRA mutated GIST cases that may still benefit from imatinib mesylate (Gleevac), an inhibitor of the kit tyrosin kinase. In addition, Dog1 immunoreactivity is seen in fewer cases of mesanchymal, epithelial tumors and melanomas when compared with c-Kit. The use of this highly sensitive and specific novel marker will increase the accuracy of GIST diagnosis.
Catalog #	D8079-35
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 1:50. Formalin/paraffin for 20 min at RT. Staining of formalin fixed sections require heat induced antigen retrieval using EDTA, pH 8, heating to 98°C for 20 min
	Optimal dilutions to be determined by the researcher.
Positive Control	Gastrointestinal stromal tumors
Cellular Localization	Cytoplasmic & Membranous
Molecular Weight	78kD
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	10A20
Host	Rabbit
Source	Human
Form	Tissue culture supernatant with 15mM sodium azide.
Purity	Supernatant
Immunogen	Synthetic peptide of human Dog1
Specificity	Recognizes human Dog1
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.