Anti -Dok2, p56, phosphorylated (Tyr351) (Docking Protein, Downstream of Tyrosine Kinase,
Dok-R, p56Dok2, FRIP, rasGAP-associated docking protein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Docking proteins are substrates of tyrosine kinases and function in the recruitment and assembly of specific signal transduction molecules. There are five members in p62dok family including p62Dok (Dok-1), p56Dok-2 (Dok-2, or DoK-R), Dok-3, Dok-4 and Dok-5 (1–3), which are characterized by the presence of an amino-terminal PH domain, a central PTB domain and numerous potential sites of tyrosine phosphorylation. Tyrosine phosphorylation of p56Dok-2 occurs upon stimulation of cells with a variety of stimuli or in cells transformed by oncogenic tyrosine kinases such as v-Src and Bcr-Abl (3–5). Very little is known about p56Dok-2 function and the role of phosphorylation in the modulation of these functions. Based on the presence of several signaling domains (PH, PTB domain, tyrosine residue and proline-rich regions), it has been proposed that they act as docking proteins that link RTKs to signal transduction pathways. p56Dok-2 has been proposed to be a negative regulator of cytokine-induced proliferation in T cells (5). It was demonstrated that phosphorylated Tyr351 mediates an association with the SH2 domain of Nck (4). | | | Catalog # | D8080-02A | | Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok. Human DOK1 is a 481 amino acid protein. | | Applications | Suitable for use in Western Blotting. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol | | Purity | Protein A and peptide affinity chromatography | | Immunogen | Synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding tyrosine 351 of human p56Dok-2 | | Specificity | Detects endogenous levels of p56Dok-2 only when phosphorylated at tyrosine 351. Does not crossreact with other tyrosine phosphorylated p62Dok family members. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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