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You are here:Home » Antibodies » Abs to Signal Transduction » Anti -Dok2, p56 (Docking Protein, Downstream of Tyrosine Kinase, Dok-R, p56Dok2, FRIP, rasGAP-associated docking protein)

Anti -Dok2, p56 (Docking Protein, Downstream of Tyrosine Kinase, Dok-R, p56Dok2, FRIP,
rasGAP-associated docking protein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
The binding protein Dok-2 is also known as p56dok-2. It is a 56kD tyrosine phosphorylated protein, purified and cloned from p210bcr-abl expressing cells, is able to bind to p120RasGAP. There is 35% identity to Dok-1 (p62dok-1). Dok-2 is a rasGAP-associated docking protein expressed mostly in hematopoietic cells. Dok-2 is phosphorylated upon activation of many protein tyrosine kinases (PTKs), including those coupled with cytokine receptors and oncogenic PTKs like Bcr-Abl. Loss of Dok-1 and/or Dok-2 in a knock out mice model carrying the bcr-abl gene resulted in progression of a myeloproliferative disease resembling human chronic myelogenous leukemia. The mouse model suggests that both Dok-1 and Dok-2 suppress leukemia as negative regulators of cytokines responses as well as maintain myeloid homeostasis.
Catalog #D8080-02C
ApplicationsSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000. Detects ~60kD band.
Immunocytochemistry:1:300
ELISA: 1:100,000 (peptide).
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide before the addition of 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa393-412 with added cysteine (C-ASGWQPGTEYDNVVLKKGPK) from the C-terminus of human p56dok2.
SpecificityRecognizes human p56dok2 (~55-60kD). Species Crossreactivity: May crossreact with mouse p56dok2 RasGAP-binding protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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