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Anti -Dok2, p56 (Docking Protein, Downstream of Tyrosine Kinase, Dok-R, p56Dok2, FRIP, rasGAP-associated docking protein)
Anti -Dok2, p56 (Docking Protein, Downstream of Tyrosine Kinase, Dok-R, p56Dok2, FRIP,
rasGAP-associated docking protein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IH |
|
| The binding protein Dok-2 is also known as p56dok-2. It is a 56kD tyrosine phosphorylated protein, purified and cloned from p210bcr-abl expressing cells, is able to bind to p120RasGAP. There is 35% identity to Dok-1 (p62dok-1). Dok-2 is a rasGAP-associated docking protein expressed mostly in hematopoietic cells. Dok-2 is phosphorylated upon activation of many protein tyrosine kinases (PTKs), including those coupled with cytokine receptors and oncogenic PTKs like Bcr-Abl. Loss of Dok-1 and/or Dok-2 in a knock out mice model carrying the bcr-abl gene resulted in progression of a myeloproliferative disease resembling human chronic myelogenous leukemia. The mouse model suggests that both Dok-1 and Dok-2 suppress leukemia as negative regulators of cytokines responses as well as maintain myeloid homeostasis. | | | Catalog # | D8080-02C | | Applications | Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. | | Recommended Dilutions | Western Blot: 1:1000. Detects ~60kD band. | | Immunocytochemistry:1:300 | | ELISA: 1:100,000 (peptide). | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide before the addition of 40% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to aa393-412 with added cysteine (C-ASGWQPGTEYDNVVLKKGPK) from the C-terminus of human p56dok2. | | Specificity | Recognizes human p56dok2 (~55-60kD). Species Crossreactivity: May crossreact with mouse p56dok2 RasGAP-binding protein. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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