You are here:Home
»
Antibodies
»
Abs to Binding Proteins
»
Anti -Dok3 (Docking Protein 3, DOK Like Protein, DOKL, FLJ22570)
Anti -Dok3 (Docking Protein 3, DOK Like Protein, DOKL, FLJ22570)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B |
|
| Downstream of kinase 3 (DOK3) is a 53kD (predicted) member of the DOK family of cytoplasmic adaptor proteins and is expressed in B cells and macrophages. It contains one pleckstrin homology region (aa63-179) and an IRS-type phosphotyrosine binding domain (PTB) (aa213-317). Alternate splicing generates additional isoforms with extensive N-terminal and C-terminal truncations and substitutions. Phosphorylation of tyrosine residues in DOK3 regulates its interaction with SH2 and SH3 containing proteins, leading to inhibition of signaling from immunoreceptors. Over (aa181-271), human DOK3 shares approximately 80% aa sequence identity with mouse and rat DOK3. | | | Catalog # | D8080-06C | | Applications | Suitable for use Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.25ug/ml | | Optimal dilutions to be determined by the researcher. | | Hybridoma | Myeloma cells with spleen cells from Balb/c mice. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 11D44 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a lyophilized powder in PBS with 5% trehalose Reconstitute with sterile PBS. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Recombinant corresponding to aa181-271 from DOK3 expressed in E. coli (Q7L591). | | Specificity | Recognizes human DOK3. Species Cross reactivity: Detects endogenous human DOK3 at~65kD in Western blots. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
