Dolichos biflorus (DBA, Horse gram) (HRP)

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Specifications

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.

Catalog #	D8085-20
	Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.
Molecular Weight	140kD
Source
Dolichos biflorus from Horse gram.

Concentration
~0.5mg/ml

Form
Supplied as a liquid in PBS, pH 7.2-7.4. Labeled with HRP.

Activity
4ug/ml will agglutinate human type A1 cells. 200ug/ml to agglutinate human type A2 cells.

Carbohydrate Specificity
Methyl-2-acetamido-2-deoxy-D-galactose.

Inhibitory Carbohydrate
Terminal alpha-D-Acetylgalactosamin

Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Alternate names	Horse Gram

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