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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E B IH IC

The Dopamine D2 Receptor is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. 2 isoforms are reported for the D2 Dopamine Receptor, Isoform 1 and isoform 2 which are expressed in the pituitary and brain; Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus.
Catalog #	D9000-13
Applications	Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: 40ug/ml using whole rat brain homogenate resulted in a single band being detected at ~48kD.
	Immunohistochemistry/Immunocytochemistry: 80ug/ml stains specific cells in various regions of PLP fixed rat brain sections including the Medial septum, Nuclcus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra teticulata and the Ventral regmental area.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	Not Determined
Form	Supplied as a lyophilized powder from 100mM ammonuim bicarbonate. Reconstitute with 100ul sterile PBS, 10mg/ml BSA.
Purity	Purified by precipitation, dialysis and chromatography.
Immunogen	D2s(AC-239-246-Cys247) covalently attached onto a carrier protein.
Specificity	Recognizes Dopamine Receptor, D2s, aa239-246. Species Crossreactivity: rat. Crossreacts with Dopamine Receptor, D2s (50%). Does not react with D2L Dopamine Receptor, D1 Dopamine Receptor (9-21), D1 Dopamine Receptor, D3 Dopamine Receptor (2-10), D3 Dopamine Receptor, D4 Dopamine Receptor (176-185), D4 Dopamine Receptor, D5 Dopamine Receptor (23-35), D5 Dopamine Receptor.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.