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You are here:Home » Antibodies » Abs to Neuroscience » Anti -Dopamine Beta Hydroxylase (DBH, Dopamine beta Monooxygenase)

Anti -Dopamine Beta Hydroxylase (DBH, Dopamine beta Monooxygenase)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Serum B IH IC
Dopamine-beta--hydroxylase (DBH) is the enzyme responsible for conversion of dopamine to noradrenaline. Tissue specificity consists of chromaffin granules of the adrenal medulla and synaptic vesicles of the sympathetic nervous system. It exists as both, a soluble and membrane-bound form in chromaffin granules.
Catalog #D9010-03
ApplicationsSuitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsImmunocytochemistry: 1:5000-1:10,000
Immunohistochemistry (Frozen): 1:20,000-1:50,000
Western Blot: 1:5000-1:10,000 (ECL)
Optimal dilutions to be determined by the reasearcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceRat
ConcentrationNot Determined
FormSuoplied as a liquid, 0.2% sodium azide.
PuritySerum
ImmunogenSynthetic peptide corresponding to aa43-62,
SEPPESPFPYHIPLDPEGTL of the N-terminal rat DBH (dopamine-beta--hydroxylase).
SpecificityRecognizes rat DBH. Specifically stains chromaffin cells of the adrenal medulla and DBH-containing cells of the sympathetic nervous system, including locus coeruleus and medulla oblongata. Species Sequence Homology: >85% in human, bovine and mouse DBH.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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