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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH

Dopa decarboxylase (DDC), also known as AADC, is a homodimeric vitamin B 6-dependent enzyme that catalyzes the decarboxylation of hydroxylated aromatic amino acids (Accession # P20711). DDC is expressed in the adrenal medulla and catecholaminergic neurons. It catalyzes the final step in the synthesis of the neurotransmitters dopamine and serotonin. Defects in DDC are associated with the development of schizophrenia, Parkinson's disease, and hypertension. Human DDC shares 89% aa sequence identity with mouse and rat DDC.
Catalog #	D9013-01F
Applications	Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution	Western Blot: 2ug/ml. Detects human DDC in cell or tissue extracts
	Direct ELISA: 0.5-1ug/ml; The detection limit is ~2ng/well.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	7H245
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Sf21-derived recombinant human DDC (aa1-480) Accession # P20711
Specificity	Recognizes human DDC. Species Sequence Homology: mouse and rat, 89%
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.