| The Dopamine D2 Receptor is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Two isoforms are reported for the D2 Dopamine Receptor, isoform 1 and isoform 2 which are expressed in the pituitary and brain; Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus. |
|
| Catalog # | D9016-10 |
| Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilutions | ELISA: 0.5-1.0ug/ml |
| Western Blot: 1:1000. Detected Dopamine Receptor D2 on 10ug of human brain lysates. |
| Immunoprecipitation: 1-10ug/ml. Use 100-400ug of protein. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms. |
| Immunohistochemistry (Paraformaldehyde fixed sections): 2-10ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Monkey brain tissue, rat or mouse brain lysate |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% BSA. |
| Purity | Purified by immunoaffinity chromotography. |
| Immunogen | Synthetic peptide corresponding to the cytoplasmic loop #3 (28aa) of the human D2 receptor. |
| Specificity | Recognizes human Dopamine Receptor D2 short and long form at ~50kD. No significant homology with other dopamine receptors (D1 and D3-D5). Species Crosseactivity: monkey, mouse and rat. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
