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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH

Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4). The two isoforms of D2R, D2 long (D2L) and short D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29 AA in the intracellular domain 3 of the D2 long form. It may play a role in the coupling of the receptor to G- proteins. All members of this family have similar structure and contain 7 putative transmembrane domains. A given cell or tissue may express more than one DR. Specific radioligands do not exist that can differentiate between these DR. Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions.
Catalog #	D9017-20
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1-10ug/ml. Using ECL
	ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.
	Immunohistochemistry: 2-10ug/ml
	Optimal dilutions to be determined by the researcher.
Control Peptide	D9017-22: Dopamine Receptor, D3, D3R, Human
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Purity	Purified over the antigen-agarose column.
Immunogen	A synthetic peptide sequence of 19aa from the human D3 receptor within the 3rd cytoplasmic domain (KLH).
Specificity	Recognizes human Dopamine Receptor, D3. No significant sequence homology with other dopamine receptors (D1, D2, D4 or D5). Species sequence homology: monkey, 94%;
mouse, 72%; rat, 68%
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.