| The transmembrane dopamine transporter (DAT) is located on the presynaptic nerve terminal and is responsible for terminating dopaminergic transmission by transporting dopamine from the synaptic cleft into the dopaminergic neuron (reuptake). Dopaminergic pathways have been strongly implicated in reward and addiction, motivation, alcoholism, ADHD and degenerative motor diseases such as Parkinson’s, Huntington’s and Chorea. |
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| Catalog # | D9020 |
| Applications | Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: Recognizes diffuse band at ~70-85kD on extracts (20ug total protein) from human caudate, putamen and nucleus accumbens. Not recommended for use on rat. |
| Immunohistochemistry (4% paraformaldehyde fixed tissue): 1:1000-1:10,000; Care should be taken not to over-fix tissue. Perfusion is followed by less than 90 minutes post-fixation, then cryoprotect. For use on rat tissue, a rat PAP system or ABC detection to enhance sensitivity is recommended. Recommended secondary antibodies: adsorbed anti-rat. |
| Immunocytochemistry: 1:5000-1:10,000 |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Brain (caudate, putamen and nucleus accumbens) |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a,k |
| Clone No | 2Q45 |
| Host | Rat |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a liquid. No preservative added. |
| Purity | Supernatant |
| Immunogen | N-terminus of human dopamine transporter fused to Glutathione S Transferase. |
| Specificity | Recognizes human dopamine transporter, N-terminus. Shows no crossreactivity to the closely related serotonin and norepinephrine transporters. Species Crossreactivity: monkey, mouse and rodent |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
