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You are here:Home » Antibodies » Abs to Transport Proteins » Anti -Dopamine Transporter (DAT)

Anti -Dopamine Transporter (DAT)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).
Catalog #D9020-01B
ApplicationsSuitable for use in Western Blot and Immunohistochemistry (Fr). Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
Form10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg per ml BSA and 50% glycerol
PurityPurified by immunoaffinity chromatography.
ImmunogenPeptide from the Intracellular C-terminal region of Human Dopamine Transporter (DAT), conjugated to keyhole limpet hemocyanin (KLH).
SpecificitySpecific for the ~88k DAT protein in Western blots of SDS-solubilized human and mouse striatal samples and in IHC applications with formaldehyde-fixed human and monkey (Macaque) brain sections. Species Crossreactivity: This antibody recognizes DAT in human, mouse, and monkey striatum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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