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You are here:Home » Antibodies » Abs to Transport Proteins » Anti -Dopamine Transporter (DAT)

Anti -Dopamine Transporter (DAT)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Dopamine is an endogenous catecholamine that influences many cellular activities. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based on the aa identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B and D5) or D2-like (D2, D3 and D4). The two isoforms of D2R, D2 long (D2L) and short (D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular domain 3 of the D2 long form. All members of this family have similar structure and contain 7 putative transmembrane domains. A given cell may express more than one DR. Specific radioligands do not exist that can differentiate between these DR. Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions. Dopamine transporter (DAT) is plasma membrane transporter for the termination of dopaminergic neurotransmission by its high affinity Na+ and Cl- reuptake of dopamine into presynaptic terminals. This protein is the target of psychomotor stimulants such as amphetamines or cocaine. DAT (human 620aa; rat/mouse 619aa glycoprotein) is predicted to contain 12 transmembrane domains with N- and C-termini in the cytoplasm.
Catalog #D9020-18
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000-1:5000 (ECL)
ELISA: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptide corresponding to 20aa within the C-terminus, cytoplasmic domain of human DAT, conjugated to KLH. Species Sequence Homology: monkey; 100%; rat; 94%; mouse; 90%
SpecificityRecognizes human Dopamine Transporter (DAT). No significant sequence homology is seen with other transporters.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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