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You are here:Home » Antibodies » Abs to Transport Proteins » Anti -Dopamine Transporter (DAT)

Anti -Dopamine Transporter (DAT)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
The dopamine transporter (DAT) is a 70-80kD member of the Na + -neurotransmitter symporter family of transmembrane (TM) proteins. DAT plays a crucial role in the synaptic clearance of dopamine (DA). It mediates the uptake of dopamine by the presynaptic terminal, thus limiting the strength of the dopamingeric response. One molecule of DA is accompanied by two Na + and one Cl-ion. Molecules such as amphetamine both competitively inhibit DA uptake, and induce DA release through the DAT, increasing the rewarding property of DA. Human DAT is a 620aa, 12 TM phosphoglycoprotein with an N- and C-terminal cytoplasmic domain. It exists as a disulfide-linked oligomer on the cell surface. Phosphorylation of the N-terminus (S7/12) promotes DA release. The C-terminus binds CaMKII, as well as Hic5, Pick1, and synuclein which regulate receptor trafficking and expression. There is an extended 71aa extracellular (EC) loop between TM segments 3 and 4. Glycosylation at this site is necessary for oligomer expression on the cell membrane. The human 42aa DAT C-terminus is 93% aa identical to the mouse DAT C-terminus. The human EC loop is also 93% aa identical to the rhesus monkey EC loop.
Catalog #D9020-19A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000. Sufficient for 10 mini-blots.
Immunohistochemistry: 1:1000. Frozen sections
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the EL2 region of human DAT, conjugated to KLH.
SpecificitySpecific for the ~88kD DAT protein in Western blots of SDS-solubilized human striatal samples and in IHC applications with formaldehyde-fixed human and monkey brain sections.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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